Main Article Content
Blood Cancer-in the shape of carcinogenesis, is worldwide recognized, as a recent time catastrophe. Its unique capability of sustaining its dormancy, vulnerabilities, of drug screening methodologies, and most importantly therapeutic resistance of tumour affected stem cells(due to the redundancy of CD-133+ cells against Radio therapeutic treatment procedure and existence of MDR-1, and ALDH-1 proteins in drug screening methodologies)  has transformed blood cancer, as hardly curable. To face this challenge; Organoids are figured out to be a possible solution. From a researcher’s point of view organoids are generally 3D structured in (vivo) clusters of stem cell molecules , showcasing bio-active capabilities. However, the lower success rate of organoids, bespeaking its initial stages of preclinical level of studies. In addition, most of these models & their implications just only been limited to in (vivo) principles and various forms of cancer exemplifying; Blood lymphoma. Interestingly, some recent milestones of organoids in different research models on metastasis reflect the glimpses of hopes. At this present study, we have worked on organoids and their possible involvement in blood cancer. We have emphasized on organoid modelling both in (vivo) and in (vitro) cell culture, which are some excellent sources for cell analysis. Presently, we have established a model where a Nano-sized in (vitro-vivo) cell clustering of organoids with an MRI scanning technique been utilized to build a more precise and useful therapeutic tool. This innovative approach would help us to identify the tumours that will not respond to any conventional therapies. Also in our studies the organoids have shown active cellular level of immunomodulation, leading to a proper signal transduction. As a consequences, this revolutionary model creates opportunities for a better outcome in terms of diagnostics and therapeutics.
[PMCID: PMC2739000][NIHMSID: NIHMS123201] [PMID: 18539962]
Enusa Ramani, Economic assessment of diagnostic systems against neglected diseases In African children under five years of age.
Real Choices in health & wellness, The Bio Regulator Company.
Robyn stoller, 7 Facts you need to know about blood cancers, National Foundation for Cancer Research, 13th September, 2017.
Kristi Baker, Nagle PW. Seminars in cancer biology, Patient-derived tumor organoids for prediction of cancer treatment response; 2018.
Xu et al. Journal of Hematology & Oncology. Hanxiao Xu, Xiaodong Lyu, Ming Yi, Weiheng Zhao, Yongping Song, Kong Ming Wu. Organoid technology and applications in cancer research. 2018; 11:116.
Bone Marrow, National Cancer Institute.
A three-dimensional tissue culture model to study Primary human bone marrow & its malignancies.
Histology department,Faculty of Medicine, Cairo University.
Hematopoietic stem cells, bioexplorer.net.
Evaluating the varied appearances of Normal & Abnormal Marrow, RAD Source.
What is an MRI (Magnetic Resonance Imaging)? , Live Science.
Makena MR, Ranjan A, Thirumala V, Reddy AP. Cancer stem cells: Road to therapeutic resistance and strategies to overcome resistance.
Md. Shaifur Rahman, Hossen Mohammad Jamil, Naznin Akhtar KMT, Rahman, Rashedul Islam SM. Asaduzzaman stem cell and cancer stem cell: A tale of two cells. 2016;3(02):97-108.
[Published: Jun 24, 2016]
Manuela Unbischek, Helena Rannikmae, Thomas Foets, Ktahrina Ravin, Marko Hyvonen, Marc de la Roche. Organoid culture media formulated with growth factors of defined cellular activity; 2019.
MERCK, DMEM/F12 Media
Scien Cell Research Laboratories, inc.
Esther Landhuis. Tumor organoids may speed Cancer treatment. 2019;195(1):9.
GE Healthcare, Discovery MR750-60cm, Lease MRI Scanner
Adrian Herper, MD, PhD, Executive vice Adrian Herper, MD, PhD, Executive vice president and Chief medical officer, Eagle pharmaceuticals, 12/14/ 2016, 7:30 am, ‘Nanotechnology & the New Generation of Injectable Medicines’
Syringe, Shutter stock
Hera, in vivo solutions available or in development.
50 ml centrifuge tube, Pluimate-Pluriselect.
Huayu yang, Lejia sun, Meixi liu, Yilei Mao; Patient derived organoids: a promising model for personalized cancer treatment; 2018.
Wnt signalling pathway, Wikipedia.
Small Molecules, A 83-01, Biogens (A peproTech brand)
Hedgehog pathway inhibitor, Wikipedia.
American Society of Hematology
Acute lymphoblastic leukemia. Wikipedia.
Acute Myeloid leukemia. Wikipedia.
Chronic Lymphocytic leukemia. Wikipedia.
Chronic Myelogenous leukemia. Wikipedia.
Non-Hodgkin Lymphoma. Wikipedia.
Hodgkin Lymphoma. Wikipedia.
Multiple Myeloma. Wikipedia.
Modelling cancer immunomodulation using epithelial organoid cultures Yotam E. Bar Ephraim, Kai Kretzschmar, Priyanca Asra, Evelien de Jongh, Kim E. Boonekamp, Jarno Drost, Joost van Gorp, Apollo Pronk, Niels Smakman, Inez J. Gan, Zsolt Sebestyén, Jürgen Kuball, Robert GJ, Vries, Hans Clevers.
Organoids provide an important window on inflammation on cancer; 2018.