Synergistic Effect of 5-Fluorouracil Combined with Naringin in MDA-MB-231 Human Breast Cancer Cells

Thangavel Muthusamy *

Faculty of Allied Health Sciences, Meenakshi Academic of Research and Education, MAHER (Deemed to be a University), West K.K. Nagar, Chennai, 600078, Tamil Nadu, India and Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai, 603103, Tamil Nadu, India.

L. Roshini Yadav

Faculty of Allied Health Sciences, Chettinad Academy of Research and Education (CARE), Kelambakkam, Chennai, 603103, Tamil Nadu, India.

Satish Ramalingam

Department of Genetic Engineering, School of Bio-engineering, SRM Institute of Science and Technology, Kattankulathur, Kanchipuram, 603203, Tamil Nadu, India.

Ilangovan Ramachandran

Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, 600113, Tamil Nadu, India.

*Author to whom correspondence should be addressed.


Abstract

Antimetabolite established as a successful therapeutics for advanced-stage breast cancers, but has recurrently shown to exhibit major side effects, which restrict drug therapy interventions. The most widely used chemotherapeutic agent is 5-Fluorouracil, however the recent report suggests that its antineoplastic activity is limited due to drug resistance developed by the cancer cells. The resistance results in the decrease in efficacy of 5-FU to improve either invasive disease-free survival or overall survival. In order to enhance 5-Fluorouracil mediated cytotoxicity, we have attempted the combination of the natural compound Naringin with 5-Fluorouracil. The combination of 5-Fluorouracil and Naringin inhibited the proliferation of MDA-MB-231 breast cancer cells determined using MTT assay. Importantly, combined treatment showed synergistic enhancement of breast cancer cell cytotoxicity. We next confirmed the cytotoxicity profile of the combination 5-Fluorouracil and Naringin in MDA-MB-231 breast cancer cells with trypan blue-based cell viability assay and determined that the IC50 value is 80 μM. When 5-Fluorouuracil and Naringin incubated there is a 7–10-fold increase in cytotoxic effect on breast cancer cells. These results demonstrates that application of naringin as an adjuvant treatment during 5-Fluorouracil administration might enhance the chemotherapeutic efficacy of 5-Fluorouracil.

Keywords: 5-fluorouracil, Naringin, cytotoxicity, MDA-MB-231 breast cancer cells.


How to Cite

Muthusamy, T., Yadav, L. R., Ramalingam, S., & Ramachandran, I. (2020). Synergistic Effect of 5-Fluorouracil Combined with Naringin in MDA-MB-231 Human Breast Cancer Cells. International Research Journal of Oncology, 3(2), 104–118. Retrieved from https://journalirjo.com/index.php/IRJO/article/view/58

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